The CacoReady 2 permeability Assay Kit is a ready-to-use cell-based assay for rapid Caco-2 in-vitro assessment of drugs’ ability to cross the intestinal epithelium.
CacoReady is designed to aid researchers and pharmaceutical companies in their evaluation of the oral absorption efficiency, oral bioavailability, and oral toxicity of drugs.
The Assay Kit has been proven to produce consistent and reliable results in multiple experiments performed by various users. Therefore, one can rest assured that the results are reproducible. CacoReady 2 is the premier in Cell Permeability Testing.
It proves cost-effective as it allows researchers to utilize the time they would spend creating their own Assay Kit to instead further their research.
The contents are designed for easy handling, and the process of using it is highly intuitive ensuring one will not have to waste time learning to use it. The CacoReady Assay Kit is an ideal tool for drug discovery and development.
The CacoReady Assay Kit comes in two sizes:
This Assay Kit has a wide range of applications.
The CacoReady Assay Kit meets the four evaluation results required by the FDA, found here, for compounds evaluated from data generated by the CacoReady kits. These requirements are as follows:
Avail discounted evaluations now, and rest assured with our extensive testing experience that our results meet or exceed your expectations.
|CacoReady Kit FORMATs||Product Numbers|
|CacoReady 24 Transwell (Costar)||KRECE-CCR01|
|CacoReady 24 Transwell (Millipore)||KRECE-CCR03|
|CacoReady 24 Well (individual transwell)||KRECE-CCR04|
|CacoReady 96 Transwell (Costar)||KRECE-CCR50|
|CacoReady 96 Transwell (Millipore)||KRECE-CCR52|
|CacoReady 24 Well (without transwell)||KRECE-CCR02|
|CacoReady 96 Well (without transwell)||KRECE-CCR51|
CacoReady allows conducting permeability assays (apical to basal or basal to apical) of established or unknown compounds to predict their absorption and interaction with membrane-associated proteins (MDR1-Pgp and BCRP)
Yes – Since these kits utilize ReadyCell’s Proprietary shipping medium, these Assay Kits are shipped at room temperature anywhere in the world.
These Cell-Based Assay Kits are made-to-order. All new orders are prepared on the following Monday, then shipped 14 days later, and received on day 16 or 17.
To use an Assay Kit with rapid Caco-2 in-vitro to assess a drug’s ability to cross the intestinal epithelium, first, you need to obtain the Cell-Based Assay, which comes with 24 or 96 insert-integrated permeable supports. They are seeded with differentiated and polarized Caco-2 barriers on polycarbonate microporous filters.
Next, you will prepare the drug sample and add it to the Assay Kit. The drug will then interact with the Caco-2 barriers and cross the intestinal epithelium, allowing for Cell Permeability Testing.
The kit provides four evaluation results pertaining to the compounds evaluated from data generated by the CacoReady kits, which the FDA requires: permeability assay, P-gp interactions, BCRP interactions, and toxicology studies.
After incubation, the Assay Kits are ready to assay with your compounds on day twenty-two. The results are highly reproducible, and the assay kit is user-friendly and easy to handle, making it adaptable to automation.
By using the Cell-Based Assay Kit, you can evaluate the oral absorption efficiency, oral bioavailability, oral toxicity, and mechanisms involved in oral and intestinal absorption of the drug. The Assay Kit is suitable for research on new delivery systems and studies of drug transporters and drug-drug interaction.
There are three main types of Assay Kits: colorimetric, fluorometric, and luminescent.
Colorimetric assays use a color change to indicate the presence of the molecule of interest, while fluorometric assays use fluorescence to detect the molecule.
Luminescent assays, on the other hand, use light emission to measure the amount of the molecule.
No matter which type of assay you need, it’s important to choose a high-quality Assay Kit that provides accurate and reproducible results. At [Company Name], we offer a wide range of Assay Kits for various applications, including drug discovery, disease research, and more. Browse our selection today to find the right Assay Kit for your needs.
An Assay Kit is a laboratory tool used to detect and quantify a specific biological, chemical, or physical property in a sample. Assay Kits are commonly used in research and diagnostics to identify and measure analytes, such as proteins, nucleic acids, or small molecules. The Assay Kit typically includes a set of reagents, instructions, and standards that allow researchers to perform the assay with accuracy and precision. The CacoReady is a Cell-Based Assay designed specifically for testing drug transporter events.