P-glycoprotein (MDR1) is a critical efflux transporter in the field of drug development and pharmacology. Here are some potential applications for a product like PreadyPort MDR-1:
As P-glycoprotein can affect drug absorption, distribution, metabolism, and excretion, understanding its interaction with a new drug candidate can be essential. This helps to predict the drug’s behavior in the body and can inform dosage and safety considerations.
MDR-1 plays a role in the metabolism of many drugs. Studying how a new drug interacts with MDR-1 can help identify potential drug-drug interactions, which could lead to adverse effects if two incompatible drugs are taken together.
By studying the interaction of drugs with MDR-1, researchers can create pharmacokinetic models that predict how a drug will be absorbed, distributed, metabolized, and excreted from the body.
Overexpression of MDR-1 can cause multidrug resistance in cancer cells, limiting the effectiveness of chemotherapy. Researching this mechanism could aid in the development of more effective treatments.
|PreadyPort-MDR1 Kit FORMATs
|PreadyPort-MDR1/CTRL 1:1 (24-Multiwell)
|PreadyPort-MDR1/CTRL 1:1 (96-Multiwell)
|PreadyPort-MDR1/CTRL Custom setup (96-Multiwell)
MDR1 stands for “Multidrug Resistance Protein 1.” It is a gene that encodes for an important protein known as P-glycoprotein (P-gp) or ATP-binding cassette sub-family B member 1 (ABCB1).
MDCK (Madin-Darby Canine Kidney) cells are an epithelial cell line derived from the kidney of a normal adult female cocker spaniel. MDCK cells are widely used in drug permeability studies as an in vitro model system for assessing the transport properties of drug candidates across biological membranes, such as the blood-brain barrier and other epithelial and endothelial barriers.
P-glycoprotein (P-gp), encoded by the MDR1 (Multidrug Resistance Protein 1) gene, is an efflux transporter that plays a crucial role in drug disposition. It is a member of the ATP-binding cassette (ABC) transporter family and is expressed in various tissues, including the intestine, liver, kidney, and the blood-brain barrier.
Madin-Darby Canine Kidney (MDCK) cells are an epithelial cell line derived from the kidney of a normal adult female cocker spaniel. They have been widely used as an in vitro model in various biological studies, including drug permeability and transport research. Some key characteristics include epithelial morphology and tight junction formation.
MDR1 P-glycoprotein is crucial in the body as it plays a significant role in the pharmacokinetics of many drugs. This protein is involved in the transportation of various molecules across the cell membrane, including many drugs. It’s particularly important in cancer treatment because its overexpression can lead to multidrug resistance in cancer cells. In veterinary medicine, some breeds of dogs have a mutation in the MDR1 gene, making them more sensitive to certain drugs, which can lead to severe adverse reactions.
The MDR-1 Testing Kit measures the presence or absence of mutations in the MDR1 gene, which codes for P-glycoprotein. This gene plays a critical role in how a body metabolizes certain medications. In animals (specifically certain dog breeds), a mutation in this gene can lead to increased sensitivity to various drugs.
PreadyPort MDR1 kit contains MDCKII cells transfected with the ABCB1 gene to overexpress the multidrug resistance protein 1 (MDR1), a membrane transporter of considerable clinical importance, to evaluate drug-transporter interactions in preclinical stages1.
PreadyPort is delivered in a 24-well/plate format with a unique Shipping Medium (a gel-like cell culture medium) established by READYCELL which enables the transport of cells at room temperature and in a ready-to-use format.
Once the samples are returned to the lab, standard laboratory equipment should be adequate to process them. The equipment required includes a PCR machine and other materials for DNA extraction, amplification, and sequencing.